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Table of Contents
Part 3: Tractography
Diffusion weighted imaging is an important development in MRI that gave clinicians and researchers the ability to identify and measure white matter non-invasively. But it also just happens to be one of the most visually beautiful types of scientific imaging (IMHO). The rest of this lab is aimed at getting you more comfortable with visualizing white matter tracts, but also to just have fun and create some awesome looking images.
In order to simplify things and move you through this lab a bit quicker, we will skip the “Single Subject” section of the lab.
Skip ahead to the Population Template section of the lab.
Visualizing Tracts with DSI Studio: Single Subject
The sample data for this section are located in ~/Desktop/input/dti-sample. The files include:
data.nii.gz- DWI data (the preprocessing applied to these data is summarized below)bvalsandbvecs- 60 directions, b1000T1_brain.nii.gz- Extracted T1-weighted brain
Preprocessing
- Denoising
- Gibbs ringing artifact
- Susceptibility artifact
- Eddy current distortions
- Subject motion
In this section we will use DSI-Studio program to visualize white matter tracts.
It is best to not use the macos “dark mode” setting when using DSI-Studio. If you never changed this setting, then you have nothing to worry about. If you did set your computer to use Dark mode, then you should set it back to Light mode for the rest of this lab.
Getting Started
1. Open DSI-Studio by clicking on the icon in your dock. 
- If a license agreement pops up, select
Accept and Sign-In
If you don't see the icon in your dock, you can open it from your Applications directory.
Preparing a single subject
1. Click on the Tools tab.
2. Select R1: Linear Registration
- Select the
T1_brain.nii.gzfile - Then select the
data.nii.gzfile
3. Check the quality of the registration by using the sliders to move through slices, and using the Click to switch views button.
- Unless something look horribly wrong, select
OK
4. Select the Tractography tab.
5. Select Step T1: NIFTI to SRC …
- Select the
data.nii.gz- The
bvecandbvalvalues will load automatically. - Click
Okin the popup window indicatingSRC file created
6. Select Step T2: Reconstruction …
- Select the
data.szfile - Select Run Reconstruction in the bottom-right corner of the window that opens up.
- Click
Okin the popup window indicatingFIB file created - Close the reconstruction window
7. Select Step T2: Fiber Tracking
- Select the
data.dti.fzfile
Viewing a single subject
A new DTI viewing window will open (see below). There is a lot going on in this window and it will take you some time to get it figured out. There are way too many options for us to review in this lab, so I will highlight just a few, but you should mess around, press buttons, see what things do. You'll discover cool stuff. If you really lose your way, you can always close the program, reopen it, and jump to step #7 above.
There are five windows:
- The main viewing window (yellow box)
- The three sliders at the bottom will move you through the x, y, and z dimensions.
- To the right of the sliders is an option to zoom in or out.
- This brain can also be rotated in 3D by click-hold-drag.
T3a Assign Regions(red box)- Here we will add regions to filter and select the tracts we want to view.
T3b Draw Regions(blue box)- This is a fractional anisotropy map color coded by the principle diffusion direction.
- In the upper corner of this window is a zoom window (just below the little
x. Adjust that number to zoom in. If you zoom in a lot, you will see the different orientations of the principle diffusion directions. - By default, this is mirrored with the brain in the main window. Adjusting the slider will move through the slices of both brains.
- The little icons to the right of the slider let you choose axial, sagittal, coronal, or multi-view perspectives.
- Among other things, you can hand draw regions of interest (ROI)
T3c Options(cyan box)- A million different options for all of the windows
T3d Tracts(purple box)- Here you will generate and control which tracts are displayed.
1. Choose the coronal view by selecting the appropriate button underneath the T3b window (hint: it's the second one to the right fo the slider)
2. In the main window set the y-slider to 65 (from left to right, the three sliders are x, y, and z)
In the main window you should see two prominent, vertical, white-matter tracts. You should also see a prominent horizontal tract that seems to connect the two vertical tracts about two-thirds of the way up.
In window T3b you'll see that the two vertical tracts are primarily blue, whereas the horizontal tract is primarily red. Zoom in close and you'll see the length and orientation within each voxel. The two vertical tracts are the right and left corticospinal tract and the horizontal tract is the corpus callosum.
3. Click Autotrack in the T3d window.
4. Click Fiber Tracking in the T3d window.
- Pretty cool, right!?
- Spend some time playing around with the brain, rotate to see all the pretty colors and fibers. Adjust the sliders, zoom, view, options in
T3c, etc. We'll be doing some more specific things in a bit, so it's good to get as familiar with the interface as you can by just messing around.
The tracts are overlaid onto a fractional anisotropy (FA) map. You might prefer to see them overlaid on a T1-weighted hi-resolution brain. To do so, on the top menubar select Slices –> Insert Other Images… and select the T1_brain.nii.gz.
In the upper left of the main window is dropdown menu that you can now use to switch between fa and T1_brain
Visualizing Tracts with DSI Studio: Population Template
DSI description of the template data:
A group-average template was constructed from a total of 1065 scans. A multishell diffusion scheme was used, and the b-values were 1000, 2000, and 3000 s/mm2. The number of diffusion sampling directions were 90, 90, and 90, respectively. The in-plane resolution was 1.25 mm. The slice thickness was 1.25 mm. The diffusion data were reconstructed in the MNI space using q-space diffeomorphic reconstruction (Yeh et al., Neuroimage, 58(1):91-9, 2011) to obtain the spin distribution function (Yeh et al., IEEE TMI, ;29(9):1626-35, 2010). A diffusion sampling length ratio of 1.7 was used. The output resolution in diffeomorphic reconstruction was 1 mm isotropic. The restricted diffusion was quantified using restricted diffusion imaging (Yeh et al., MRM, 77:603–612 (2017)).
Getting Started
1. Open DSI-Studio by clicking on the icon in your dock.
- If a license agreement pops up, select
Accept and Sign-In
If you don't see the icon in your dock, you can open it from your Applications directory.
2. Select the FIB Template & Tractography Atlas tab.
In the Population-averaged FIB templates box you will see a list of seven different templates.
- Select
human(Note: by default,Human human_neonateis highlighted, but that's not what you want. Be sure to selecthumanat the top of the list.) - Press
Load
We won't be using any of them for this lab, but how awesome is it that you can play with the white matter of babies, and chimpanzees, and monkeys, and rodents?! I encourage you to play around with them at some point.
(the correct answer is “Very awesome!”)
A new DTI viewing window will open (see below). There is a lot going on in this window and it will take you some time to get it figured out. There are way too many options for us to review in this lab, so I will highlight just a few, but you should mess around, press buttons, see what things do. You'll discover cool stuff. If you really lose your way, you can always close the program, reopen it, and jump to step #7 above.
There are five windows:
- The main viewing window (yellow box)
- The three sliders at the bottom will move you through the x, y, and z dimensions.
- To the right of the sliders is an option to zoom in or out.
- This brain can also be rotated in 3D by click-hold-drag.
T3a Assign Regions(red box)- Here we will add regions to filter and select the tracts we want to view.
T3b Draw Regions(blue box)- This is a fractional anisotropy map color coded by the principle diffusion direction.
- In the upper corner of this window is a zoom window (just below the little
x. Adjust that number to zoom in. If you zoom in a lot, you will see the different orientations of the principle diffusion directions. - By default, this is mirrored with the brain in the main window. Adjusting the slider will move through the slices of both brains.
- The little icons to the right of the slider let you choose axial, sagittal, coronal, or multi-view perspectives.
- Among other things, you can hand draw regions of interest (ROI)
T3c Options(cyan box)- A million different options for all of the windows
T3d Tracts(purple box)- Here you will generate and control which tracts are displayed.
Plotting tracts
We'll start by plotting a couple of major white-matter tracts.
1. Switch to a coronal view by selecting the appropriate button underneath the T3b window (hint: it's the second one to the right of the slider). In both T3b and the main viewing window zoom in so that the brains fill the respective windows.
In the main window you should see two prominent, vertical, white-matter tracts. In window T3b you'll see that the two vertical tracts are primarily blue indicating that the the principle diffusion direction is superior-inferior. Zoom in close and you'll see the length and orientation of the primary diffusion direction within each voxel. The two vertical tracts are the right and left corticospinal tract which is the primary pathway that brings motor information from the brain to the body.
Let's plot these as fiber tracts.
2. Click Autotrack in the T3d window. The button will now read All.
3. Click the All dropdown menu and select ProjectionBrainstem. A new dropdown menu will appear immediately below the only you just used. If you click on it you'll see a list of prominent white matter tracts. But the first one that is selected by default, CorticospinalTractL, is the one we want, so click on the Fiber Tracking button.
4. Select CorticospinalTractR and then Fiber Tracking.
5. In the main viewing window change the brain image from qa to t1w_template
Play around with the image in the main window. Rotate it around. Try different moving through the slices of the underlay. You can also click the check box next to each slider to turn slices on/off. For example, in the image below I've turned off the x and y slices so that we get a view of the tracts ascending through the midbrain.
As you add new fiber tracts you'll probably want to hide or remove ones you've already viewed. In T3d you can simply uncheck a given tract to hide it. If you want to remove it altogether, make sure it's highlighted and click the red “X” button.
LAB REPORT Part 3
LAB REPORT Part 3
For this section of your lab report you will plot several fiber tracts (listed below). For each tract you should choose the best way to display it (i.e., orientation, underlay slices, whether you have other tracts visible, etc.). Your figures can include multiple sub-figures if you feel the best depiction requires more than one view. But this is not mandatory.
The brain tracts you should plot:
- The main body of the corpus callosum
Commissure–>CorpusCallosum–>Body
- The forceps major, which connect the occipital lobes through the splenium of the corpus callosum.
Commissure–>CorpusCallosum–>ForcepsMajor
- The left arcuate fasciculus, which is the primary fiber bundle connecting Broca's area and Wernicke's area.
Association–>ArcuateFasciculusL
- The bilateral optic radiations, which connect the lateral geniculate nucleus and striate cortex.
ProjectionBasalGanglia–>OpticRadiationLProjectionBasalGanglia–>OpticRadiationR
Plotting tracts that run through particular regions
This section is <sigh> optional. If you'd like to play around with identifying fiber tracts that run through particular regions (including ones that you create yourself) then proceed!
I suggest that you open a new template window. If you go back to the main DSI window and click Load for the Population-averaged FIB templates it'll open a new instance. You can close the one you've been workin in, or leave it open if you prefer.
1. As you did previously, change the underlay from qa to t1w_template and click Autotrack in the T3d window.
2. Click on Fiber Tracking
Unlike earlier, you're not seeing a specific tract, but rather all of the tracts in the brain. This is very pretty, but not terribly useful so after you've explored it a bit uncheck whole_brain or delete it.
3. In T3a click on the Atlas button. This will open small window.
Let's say we're interested in seeing which fibers pass through the right fusiform gyrus.
4. In the dropdown menu select FreeSurferDKT_Cortical and then right_fusiform from the list.
- Click
Add - Click
Close
In the right temporal cortex you will now see the fusiform gyrus highlighted. It kind of looks like playdoh or clay. So that's our region of interest. We now need to tell DSI what we'd like to do with that region.
5. In T3a click the dropdown menu next to right_fusiform and select ROI. This tells the software that we want to see all of the fibers that pass through the region. A description of all these options is available here.
6. Click on Fiber Tracking in T3d.
Our “lump of clay” is kind of ugly and obscures the fibers. To make it disappear unclick Region Rendering in T3c.
7. In T3a click the dropdown menu next to right_fusiform and select End, the Fiber Tracking in T3d. This tells the software that we want to see all of the fibers that end in the region (as opposed to simply passing through). Remember to uncheck the previous fiber tract that you plotted in T3d.
You can combine regions to further limit and select fibers. For example, if we select left_pars_opercularis–a subregion of Broca's area–and left_superior_temporal–home of Wernicke's area–and set both of them to ROI then the only fibers that get plotted will be those that pass through both of the areas.
Finally, you can create custom regions rather than selecting pre-existing ones from the Atlases.
8. Right-click in T3a and select New Region.
9. In T3b you can draw your own region of interest.
- After you draw your region remember to select
ROIfrom the dropdown menu before clicking Fiber Tracking